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Influence of the protectives against Varroa destructor on the colonies of bees and the environment
Marečková, Kateřina ; Vávrová, Milada (referee) ; Čáslavský, Josef (advisor)
Varroa destructor contains about six different types of mites that came from Asia and spread to Europe and gradually to the entire world. In the 70‘s of the last century the mite was identified also in the Czech Republic. The danger of the Varroa destructor is that it‘s getting into the drone´s fetus, where it is closed by the bees. Herein the mite is procreated and developed. Since 1990, the number of colonies of bees in the Czech Republic has been decreased. This is because of various reasons but one of the main causes is varroa destructor. Correct treatment of bees is directed by the regulation number 2/2007 which was released by the regional administration for South Moravia. The regulation recommends the spring, summer, autumn and winter treatment of bees by the preparations M-1 AER, FORMIDOL laminaes, GABON PA 92 and PF 90 and VARIDOL FUM and AER. All the preparations except FORMIDOL are available only on medical prescription and they are manufactured in the Bee Research Institute at Dol (Libčice nad Vltavou). This work reviews the information on the mite Varroa destructor, his origin, distribution, therapeutics and prevention. It explores the influence of the preparations used in the Czech Republic on the environment and on the bees themselves.
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Insect pollinators issues and alternative methods of control of fungal, bacterial, and parasitic diseases of bees
MRÁZ, Petr
This Ph.D. thesis is focused on the importance of insect pollinators and pointed out to their loss due to intensive agriculture. Great attention is paid to the honey bee as the main pollinator, especially to the issue of quality nutrition of bee colonies in connection with the support of detoxification of pesticides. Furthermore, the main effort is devoted to alternative possibilities of control of selected bee pathogens and research of their prevalence in the Czech Republic. The work is divided into two main parts: a detailed background research and an experimental part consisting of six subchapters with results from my own research studies. The first study deals with the effect of pollination on the qualitative and quantitative yield parameters of honeysuckle. Several pollination variants were tested and the best result in all monitored parameters was achieved by the pollination with natural pollinators. Variants of manual pollination and without pollination caused uneven maturation of fruits and lower yields. Furthermore, the diversity and abundance of pollinators in the vicinity of this crop were observed and the most suitable pollinators identified which appear to be bumblebees and the honey bee. The second study deals with the impact of agricultural intensity on the diversity and abundance of pollinators in the landscape. Localities with organic and conventional management regimes were compared. Significantly higher diversity and abundance of pollinators was recorded in the locality managed by organic farming. In addition, the contamination load of pesticide residues in bee's body was also monitored. Residues of several pesticides were detected at the site with conventional agriculture, while none of them were detected at the site with organic farming. The third study examines the effect of nutrition, specifically phenolic substances, on the ability of bees to detoxify the pesticide to which they have been exposed. In the experiment, the bees in the cages were fed with a mixture of selected polyphenols, commonly found in pollen, and the pesticide thiacloprid. Mortality and daily feed consumption were monitored for 14 days, and the expression level of detoxification genes was analyzed at specified intervals. Phenolic substances have been shown to have a positive effect on the lifespan of intoxicated bees, as well as higher feed consumption, which may indicate an increased need for these substances. In contrast, the increased expression of detoxification genes was not confirmed. Other studies focus on honey bee pathogens. One of them monitors the occurrence and prevalence of selected major bee pathogens in the Czech Republic and compares different types of habitats, such as urban areas, agriculturally intensively cultivated areas and protected natural areas. Surprisingly, the most often detected pathogen was Lotmaria passim. From the viral diseases, the highest rate of occurrence had DWV complex and ABPV. In general, more eukaryotic pathogens were found in cities and agricultural landscapes. On the contrary, more viral diseases were recorded in the protected natural area. The fifth subchapter consists of 4 publications and deals with the use of essential oils to control the Varroa destructor mite and the entomopathogenic fungus Ascosphaera apis. The first publication compares the growth and development of the fungus A. apis on different culture media and proposes a new medium with the addition of bee brood, on which the greatest sporulation was recorded. Another 2 publications deal with the fungicidal effect of selected essential oils in laboratory conditions. The best results were shown by essential oils of thyme, cedar wood, cloves and cinnamon. The fourth publication deals with the acaricidal effect of selected essential oils on the V. destructor mites and at the same time evaluate the toxicity of these oils to adult bees. Based on these results, essential oils with the highest LD50 to bees / LD50 to mites ratio (sele
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Technické a alternativní metody k šetrnému tlumení varoózy
NOVOTNÝ, Josef
Varroosis is a disease afflicting beehive on every continent except for Australia. Since 2000 the disease is known to be caused by the Varroa destructor mite. This thesis' first goal was to summarize information about this parasite and the methods limiting its impact. The second goal was to prepare and conduct a test verifying the effectiveness of the ultrasound method, which was carried out with the Varroa-Killer-Sound device. In order to enable the analysis, bee samples from two hives were chosen and those were then tested for the quantity of Varroa destructor females using the sugar shake method. The first sample group consisted of bees treated with the ultrasound method, the latter were control beehives, which were not experimentally treated. Furthermore, other factors were continually measured: temperature, relative humidity, and dew point of the external environment. The results of the research were analyzed by the STATISTICA 12 program.
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The differences in the virome of different populations of honey bee (Apis mellifera)
Kadlečková, Dominika ; Tachezy, Ruth (advisor) ; Votýpka, Jan (referee)
European honey bee (Apis mellifera) is major pollinator for agriculture and vital for food production. Large number of viruses infecting A. mellifera have been discovered over the years, but it isn't yet known if they are pathogenic for their host. However, presence of non-viral pathogens like Varroa destructor can greatly increase their virulence and have fatal consequences for the colony. The aim of this study was to test and verify robustness of the method for virome detection on healthy honey bees from the Czech Republic. Last but not least we aimed to detect non-viral parasites and correlate their presence with detected viruses. We have successfully identified large number of viral sequences from different viral families. Viral composition was found to be influenced mainly by colony from where the honey bees were collected. That was mainly given by a large amount of bacteriophages in the samples. However, analysis of individual viruses, known to infect honey bee, indicated that viral prevalence and viral loads of specific viruses is quite different among individual honey bees from the same colony. Interestingly we were able to find highly diverse Lake Sinai viruses. We were able to observe correlations either between individual viruses or viral other non-viral pathogens. Further analysis is...
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Study of physiology and searching of proteomic tools useful in suppression and detection of Varroa destructor
Holenková, Martina ; Erban, Tomáš (advisor) ; Titěra, Dalibor (referee)
This work is focused on the study of physiology and proteome of the the mite Varroa destructor and on comparison with the honeybee Apis mellifera. Varroa is currently a major problem for beekeeping, because infects most of the colonies. The control of the mite can not be done without residues both in the hive and for example in the honey or other resources used by the man. Another problem can also be the simultaneously discussed issue of the connection with the Colony Collapse Disorder. The internal anatomy of V. destructor was studied by using paraffin histology. On histological sections stained using hematoxylin and PAS was possible to observe the mite digestive system, but also egg, ovaries or brain. The primary aim of this study was to identify the proteins of mite V. destructor and bee A. mellifera as a host of this parasite. For the electrophoretic separation was used two- dimensional gel electrophoresis, where the second dimension was carried out using 12% and 15% SDS-PAGE. The most abundant spots were selected for analysis using MALDI TOF/TOF mass spectrometry. The most abundant protein identified in samples of V. destructor was hexamerin, arginine kinase or hemelipoglycoprotein precursor. Hexamerins were also identified as the major proteins in the pupae samples. On the contrary the main...
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The differences in the virome of different populations of honey bee (Apis mellifera)
Kadlečková, Dominika ; Tachezy, Ruth (advisor) ; Votýpka, Jan (referee)
European honey bee (Apis mellifera) is major pollinator for agriculture and vital for food production. Large number of viruses infecting A. mellifera have been discovered over the years, but it isn't yet known if they are pathogenic for their host. However, presence of non-viral pathogens like Varroa destructor can greatly increase their virulence and have fatal consequences for the colony. The aim of this study was to test and verify robustness of the method for virome detection on healthy honey bees from the Czech Republic. Last but not least we aimed to detect non-viral parasites and correlate their presence with detected viruses. We have successfully identified large number of viral sequences from different viral families. Viral composition was found to be influenced mainly by colony from where the honey bees were collected. That was mainly given by a large amount of bacteriophages in the samples. However, analysis of individual viruses, known to infect honey bee, indicated that viral prevalence and viral loads of specific viruses is quite different among individual honey bees from the same colony. Interestingly we were able to find highly diverse Lake Sinai viruses. We were able to observe correlations either between individual viruses or viral other non-viral pathogens. Further analysis is...
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Physiological changes during honeybee (Apis mellifera) ontogenesis and between castes
Martišková, Ester ; Erban, Tomáš (advisor) ; Čapková Frydrychová, Radmila (referee)
This work is focused on describing the individual differences in physiology of honeybee Apis mellifera during its development. It also compares the differences between the individual caste. A lot of complex rebuilding of the whole organism takes place during the development of Apis Mellifera. Because of that, especially in the early stages, it is important to ensure a great supply of energy. Larvae need to acquire an abundant source of nutrients, so they can grow fast and have a sufficient storage for the pupal stage, during which the new body reorganization takes place. Newly emerged bees then need to quickly get accustomed to the new environment a assume their role in the hive. Queens need a constant supply of energy, so they can lay eggs for as long as possible. Drones focuse mainly on mating with the queen. The life of Apis mellifera is also influenced by other factors, primarly by numerous diseases and pathogens. The results of this work can give a comprehensive idea about the complex development of an individual bee and give a of further research. There are still a lot a lot of unanswered and unexplored realities in biology of bees, that could a topic of study and help us better understand their importance for nature.
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The most common diseases of bees keeped in Czech Republic
DURČANSKÝ, Pavel
This thesis deals with the most common disease occurring in bees kept in the Czech Republic. It focuses mainly on the Varroa destructor mite. Within individual diseases and foetus as well as adult-bee infections, I have observed the causes of occurrence of non-infectious diseases, their progress and possible ways of treatment. Furthermore, I describe infections that can be of viral, bacterial, fungal, and parasitic origin. Here I deal with the description of the originator, clinical symptoms, progress of the disease, spreading, ways of treatment and possible preventative measures.
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Parasitic bacteria Arsenophonus in honeybee and its parasite Varroa destructor
Hejdánková, Sylvie ; Hubert, Jan (advisor) ; Smrž, Jaroslav (referee)
Arsenophonus is vertically and horizontally transmitted parasitic bacteria and strengthens its transfer through phenomenon called son killing. Arsenophonus has been detected in the arthropod hosts, insects, ticks and the garden spider Araneus diadematus. The aim of this study is detection parasitic bacteria Arsenophonus in honeybees and its parasite Varroa destructor. We find out that bacteria Arsenophonus is present in both hosts and it is identified as Arsenophonus nasoniae. Detection of bacteria Arsenophonus is discovered for the first time in Varroa destructor mites. We proved that the frequency of bacteria Arsenophonus in mites Varroa destructor is significantly higher than in honeybees. This study shows that the mite Varroa destructor could act as a vector for transmission parasitic bacteria Arsenophonus among honeybees. Results of this study could lead to the future application of Arsenophonus as a biological control for the mite Varroa destructor.
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